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Separation Creek is a small coastal locality in the Shire of Colac Otway, Victoria, Australia. In the 2011 census, the population of Separation Creek was too low to separately report; however in June 2014 the Victorian Electoral Commission recorded 24 enrolled voters in Separation Creek, living in 37 properties. The Separation Creek village is situated at the mouth of the Separation Creek, on the Great Ocean Road, which follows the coast through the locality.